Review



e coli k 12 strain ja221  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    ATCC e coli k 12 strain ja221
    Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli <t>JA221</t> and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.
    E Coli K 12 Strain Ja221, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli k 12 strain ja221/product/ATCC
    Average 90 stars, based on 4 article reviews
    e coli k 12 strain ja221 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12"

    Article Title: phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12

    Journal:

    doi:

    Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli JA221 and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.
    Figure Legend Snippet: Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli JA221 and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.

    Techniques Used: Functional Assay, Clone Assay, Construct, Mutagenesis, Transformation Assay, Plasmid Preparation, Derivative Assay, Amplification

    E. coli growth with different DIPP concentrations as the sole P sources. Plates were photographed following 40 h of incubation at 34°C. (A and B) Enhanced growth of OPU mutants with DIPP as the sole P source. Five mutant strains (DB, DC, FB, FC, and FL) and the wild-type strain E. coli JA221 were used. (A) Medium containing 1 mM DIPP. (B) P-deficient and Pi-rich media. The tiny colonies of the wild-type strain on DIPP-containing plates (A) and of both the wild-type strain and mutant DB on P-deficient plates (B) could not be reproduced by photography. (C) OPU phenotype conferred by the multicopy glpT gene is independent of the gene source. The wild-type strain was transformed with the pUC19 vector carrying the PCR-amplified glpT gene from the wild-type JA221 genome (JA), the DB mutant genome (DB), the pB9 clone (pB9), or the pUC19 vector alone (control). Following transformations cells were plated onto medium containing 1 mM DIPP.
    Figure Legend Snippet: E. coli growth with different DIPP concentrations as the sole P sources. Plates were photographed following 40 h of incubation at 34°C. (A and B) Enhanced growth of OPU mutants with DIPP as the sole P source. Five mutant strains (DB, DC, FB, FC, and FL) and the wild-type strain E. coli JA221 were used. (A) Medium containing 1 mM DIPP. (B) P-deficient and Pi-rich media. The tiny colonies of the wild-type strain on DIPP-containing plates (A) and of both the wild-type strain and mutant DB on P-deficient plates (B) could not be reproduced by photography. (C) OPU phenotype conferred by the multicopy glpT gene is independent of the gene source. The wild-type strain was transformed with the pUC19 vector carrying the PCR-amplified glpT gene from the wild-type JA221 genome (JA), the DB mutant genome (DB), the pB9 clone (pB9), or the pUC19 vector alone (control). Following transformations cells were plated onto medium containing 1 mM DIPP.

    Techniques Used: Incubation, Mutagenesis, Transformation Assay, Plasmid Preparation, Amplification



    Similar Products

    e coli k 12 strain ja221  (ATCC)
    90
    ATCC e coli k 12 strain ja221
    Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli <t>JA221</t> and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.
    E Coli K 12 Strain Ja221, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli k 12 strain ja221/product/ATCC
    Average 90 stars, based on 1 article reviews
    e coli k 12 strain ja221 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli JA221 and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.

    Journal:

    Article Title: phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12

    doi:

    Figure Lengend Snippet: Functional map of phnE and glpT genes. All clones and constructs were used to transform the wild-type strain E. coli JA221 and were tested for the ability to confer vigorous growth on plates containing 1 mM DIPP as described in Materials and Methods in order to determine the functional genes. The locations and directions of transcription of the ORFs are indicated by arrows. A plus sign indicates positive growth on DIPP-containing plates, corresponding to the growth of E. coli mutant DB. A minus sign indicates negative growth on DIPP-containing plates, equivalent to the growth of the wild-type strain E. coli JA221 transformed with the control vector. Restriction enzyme cutting sites are indicated as follows: B, BglI; P, PvuII; S, PstI; N, NsiI; H, HindIII; R, EcoRI; and V, EcoRV. (A) phnE: five overlapping clones of genomic DNA obtained from E. coli mutant DB (pB1, pB3, pB12, pE2, and pF1) and three constructs derived from the pF1 clone (pFΔRL, pFΔRR, pFΔF). No sites were found for BamHI, HindIII, and PstI. (B) glpT: genomic clone (pB9) obtained from the DB mutant, a derivative construct (pqTa), and PCR-amplified regions of the glpT gene (PCRpT). No sites were found for BamHI and EcoRI.

    Article Snippet: E. coli K-12 strain JA221 (F − hsdM + hsdR lacY leuB6 ΔtrpE5 recA1 λ − ) (ATCC 33875) and the library of Alteromonas haloplanktis 214 variant 3 in E. coli K-12 strain JA221 (ATCC 37436) ( 32 ) were obtained from the American Type Culture Collection (Rockville, Md.).

    Techniques: Functional Assay, Clone Assay, Construct, Mutagenesis, Transformation Assay, Plasmid Preparation, Derivative Assay, Amplification

    E. coli growth with different DIPP concentrations as the sole P sources. Plates were photographed following 40 h of incubation at 34°C. (A and B) Enhanced growth of OPU mutants with DIPP as the sole P source. Five mutant strains (DB, DC, FB, FC, and FL) and the wild-type strain E. coli JA221 were used. (A) Medium containing 1 mM DIPP. (B) P-deficient and Pi-rich media. The tiny colonies of the wild-type strain on DIPP-containing plates (A) and of both the wild-type strain and mutant DB on P-deficient plates (B) could not be reproduced by photography. (C) OPU phenotype conferred by the multicopy glpT gene is independent of the gene source. The wild-type strain was transformed with the pUC19 vector carrying the PCR-amplified glpT gene from the wild-type JA221 genome (JA), the DB mutant genome (DB), the pB9 clone (pB9), or the pUC19 vector alone (control). Following transformations cells were plated onto medium containing 1 mM DIPP.

    Journal:

    Article Title: phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12

    doi:

    Figure Lengend Snippet: E. coli growth with different DIPP concentrations as the sole P sources. Plates were photographed following 40 h of incubation at 34°C. (A and B) Enhanced growth of OPU mutants with DIPP as the sole P source. Five mutant strains (DB, DC, FB, FC, and FL) and the wild-type strain E. coli JA221 were used. (A) Medium containing 1 mM DIPP. (B) P-deficient and Pi-rich media. The tiny colonies of the wild-type strain on DIPP-containing plates (A) and of both the wild-type strain and mutant DB on P-deficient plates (B) could not be reproduced by photography. (C) OPU phenotype conferred by the multicopy glpT gene is independent of the gene source. The wild-type strain was transformed with the pUC19 vector carrying the PCR-amplified glpT gene from the wild-type JA221 genome (JA), the DB mutant genome (DB), the pB9 clone (pB9), or the pUC19 vector alone (control). Following transformations cells were plated onto medium containing 1 mM DIPP.

    Article Snippet: E. coli K-12 strain JA221 (F − hsdM + hsdR lacY leuB6 ΔtrpE5 recA1 λ − ) (ATCC 33875) and the library of Alteromonas haloplanktis 214 variant 3 in E. coli K-12 strain JA221 (ATCC 37436) ( 32 ) were obtained from the American Type Culture Collection (Rockville, Md.).

    Techniques: Incubation, Mutagenesis, Transformation Assay, Plasmid Preparation, Amplification